Gene mutations and abnormal gene expression in ovarian cancer arising from endometriosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592480
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KITAWAKI Jo 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00204925)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ヘテロ接合性の消失 / 悪性形質転換 / 子宮内膜症から発生する卵巣癌 / 突然変異

Outline of Final Research Achievements

To investigate the genetic alterations during transformation of ovarian endometriosis into cancer, we analysed loss of heterozygosity (LOH) and mutations of tumor-related genes. Each paraffin-embedded section was microdissected to isolate the endometriotic epithelium, transitional epithelium and cancer cells. Extracted DNA was analysed for LOH events and mutations. With 13 microsatellite markers on 6 chromosomes, we detected 31 and 8 LOH events in OCEMs and in solitary ovarian endometriosis, respectively. Only two point mutations of PTEN were detected in cancer cells in one case. Our study suggested that accumulated LOH events on some chromosome loci may be involved in malignant transformation of endometriosis, while mutations in certain tumor-related genes are not.

Free Research Field

産婦人科学