Immunohistochemical localization of serotonin in the ovaries of mice with experimental polycystic ovary syndrome (PCOS)

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592492
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kansai University of Health Sciences
• Principal Investigator: HATAMURA IKUJI 関西医療大学, 保健医療学部, 教授 (80336883)
• Co-Investigator(Kenkyū-buntansha): NAKATSUKA Terumasa 関西医療大学, 保健医療学部, 客員教授 (30380752) ; SAKURAI Iori 関西医療大学, 保健医療学部, その他 (40624127)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 多嚢胞性卵巣(PCO) / セロトニン

Outline of Final Research Achievements

Polycystic ovary syndrome(PCOS) is the most common etiology of menstrual disorders and hyperandrogenism. At present, the understanding of PCOS has advanced significantl.PCO was induced by a single intraperitoneal injection of Testosteron propinate(TP)(0.1mg,dissolved in sesame oil) in 4-5 days female mice. The mice for 5 weeks after TP adminstration exhibited constantly either Estrus or Diestrus and resulted in anovulatory polycystic ovaries. These ovaries contain multiple large follicles and no corpus luteum. Its morphrogy and ovulatory failure are similar to PCOS of human. These ovaries contain multiple large follicles and no corpus luteum. Its morphrogy and ovulatory failure are similar to PCOS of human. It was subjected to immunohistochemistry of serotonin in those PCO. Expression of serotonin was strongly expressed in the later secondary follicles. It was suggested that excessive serotonin expression in ovarian caused abnormally enhance follicle maturation

Free Research Field

分子生物学