2014 Fiscal Year Final Research Report
Development of a biomarker in gynecologic cancer to predict outcome with abnormal expression of cell polarity molecules.
| Project/Area Number |
24592506
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 細胞極性制御機構 / バイオマーカー / 網羅的解析 / 転写産物 / 脱リン酸化酵素 |
|---|
| Outline of Final Research Achievements |
We performed CAGE (Cap Analysis Gene Expression) analysis to investigated transcriptome start sites (TSSs) from the perspective of both the HPV and human genomes. The result showed that the cell polarity and adhesion pathway is dramatically downregulated, and we found a novel antisense viral transcript only seen in CIN cervical cells. Using the molecular and biochemical analyses, we found that cell polarity protein hScrib regulates Ras-ERK pathway, and the activity is mediated in part through recruitment of protein phosphatase 1γ (PP1γ). Furthermore, we have explored the potential role of PP1γ in HPV-induced cervical malignancy. Then, we have found a striking concordance with cellular redistribution in pattern of expression, and this loss of PP1γ expression and redistribution in pattern of expression occurs progressively as the lesions develop. Taken together, we have concluded that PP1γ would be a novel biomarker to predict disease progression for HPV induced malignancy.
|
| Free Research Field |
婦人科腫瘍学
|
