2014 Fiscal Year Final Research Report
Metabolomic profiling in inner ear fluid by gas chromatograpy-mass
Project/Area Number |
24592547
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Otorhinolaryngology
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Research Institution | Kobe University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Daisuke 神戸大学, 医学部附属病院, 特命准教授 (60306785)
FUJITA Takeshi 神戸大学, 医学部附属病院, 助教 (90533711)
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Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Masaru 神戸大学, 質量分析センター
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 内耳 / 細胞死 / 再生 |
Outline of Final Research Achievements |
The composition and homeostasis of the inner ear fluids are important for hearing function. This study is for the first time to perform metabolome analysis of the inner ear fluid in guinea pigs cochlea using gas-chromatography/mass-spectrometry (GC/MS). Seventy-seven kinds of metabolites were detected in the inner ear fluid. Six metabolites; ascorbic acid, fructose, galactosamine, inositol, pyruvate + oxalacetic acid and meso-erythritol were significantly more abundant and nine metabolites; phosphate, valine, glycine, glycerol, ornithine, glucose, citric acid + isocitric acid, mannose and trans-4-Hydroxy-L-proline were less frequent in the inner ear fluid than in plasma. The level of ten metabolites; 3-Hydroxy-Butyrate, glycerol, fumaric acid, galactosamine, pyruvate + oxalacetic acid, phosphate, meso-erythritol, citric acid + isocitric acid, mannosea and inositol in the inner ear fluid significantly changed after loud noise exposure.
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Free Research Field |
耳科学
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