A study on the regeneration of projective neurons in the olfactory bulb after bone marrow transplantation and the reconstruction of the olfactory neural circuit

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592550
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Okayama University
• Principal Investigator: NISHIZAKI Kazunori 岡山大学, 医歯(薬)学総合研究科, 教授 (90180603)
• Co-Investigator(Kenkyū-buntansha): ORITA Yorihisa 岡山大学病院, 助教 (90362970) ; YOSHINOBU Junko 岡山大学, 医学部, 技術専門職員 (80448224)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 嗅球 / 神経細胞再生 / 嗅覚神経路 / 骨髄移植

Outline of Final Research Achievements

We have demonstrated that transplanted bone marrow-derived cells differentiate into olfactory sensory cells after transplantation of bone marrow including mesenchymal stem cells. In the present study, bone marrow-derived cells differentiated not only into microglia cells, but also rarely into mitral / turfed cells. Despite its rare frequency of trans-differentiation to mitral/turfed cells, this study is thought to show the possibility of regeneration medicine using bone marrow transplantation for central olfactory disorders at the olfactory bulb. Moreover, we have performed a series of experiments using irrigation of the olfactory bulb with N-methyI-D-aspartate, repeated intraperitoneal methimazole injections, intranasal dripping of ageing or anti-ageing reagent, and the resection of the olfactory nerve to observe the reconstruction of　neural circuit in the central olfactory pathway.

Free Research Field

耳鼻咽喉科