Molecular mechanism of barrier function in human nasal mucosal epithelia

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592580
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Juntendo University
• Principal Investigator: MIWA Masato 順天堂大学, 医学部, 准教授 (80247650)
• Co-Investigator(Kenkyū-buntansha): KAMIYA Kazusaku 順天堂大学, 医学部, 准教授 (10374159) ; IKEDA Katsuhisa 順天堂大学, 医学部, 教授 (70159614)
• Co-Investigator(Renkei-kenkyūsha): TAKAI Toshiro 順天堂大学, 医学部, 准教授 (70338375)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バリア機能 / フィラグリン / アレルギー性鼻炎 / 花粉症 / 鼻粘膜上皮 / ドライノーズ / 花粉症の初期治療 / アレルギーの先制医療

Outline of Final Research Achievements

Filaggrin (FLG) has been known as a natural moisturizing factor and barrier related protein.
We have demonstrated the expression of FLG through real time RT-PCR in human nasal mucosa excised from inferior turbinate by a noninvasive method using cotton swab. Preseasonal treatment of Japanese cedar pollinosis with antihistamine increased FLG expression in human nasal mucosa compared with postseasonal treatment.Additionally, we are investigating the alteration of FLG expression in primary cultured human nasal epithelial cells through various stimulations. After stimulation of lipopolysaccharide (LPS), poly I: C, FLG expression was diminished. On the other hand, TNFα increases FLG expression in primary cultured cells.Taken together recent reports and our original results, FLG might be a key molecule connecting innate immunity with acquired immunity in the upper airway. Further study would be expected to fix the epithelial barrier to treat and prevent AR as a barrier disorder.

Free Research Field

耳鼻咽喉科