Analysis of novel roles of T cells especially T reg cells in the pathogenesis and recurrence of Rhinosinusitis with Nasal polyps

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592584
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nippon Medical School
• Principal Investigator: PAWANKAR Ruby 日本医科大学, 医学部, 教授 (00287674)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アトピー型鼻茸 / T細胞 / ＴＳＬＰ / ＩgE / periostin / ダニ抗原 / LPS

Outline of Final Research Achievements

CD4+CD45RO+ cells was significantly more and CD4+CD25+ FoxP3(Treg) cells were significatly less in nasal polyps（NP) of atopics and non-atopics than in nasal mucosa (NM) of allergic rhinitics (AR). Levels of IL-4, IL5, IL-10 and IFN-gamma were not different, but IL-13, TSLP, periostin, IL-33, fillagrin and IgE were greaterin NP than NM especially atopics( filaggrin similar in both NPs). There was a good positive correlation between IgE and ECP, TSLP and IgE, TSLP and ECP, periostin and IL-13 and IL-33 in NP and a negative correlation between Treg cells and IgE Mite stimulated NP expressed more IL-13, IL-33, TSLP and periostin. Co-stimulation with LPS upregulated it; LPS and IL-4 upregulated TSLP, Eotaxin and RANTES. Mite stimulated T cell supernantant upregulated RANTES, Eotaxin and TSLP from epithelial cells and co- colture o fmite stimulated T cells with autologous B cells increased IgE. Reduced T reg cells and increased Th2 cells contrubute to the progression and recurance of NPs.

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