Production of soluble vascular adhesion protein-1 in diabetic retinopathy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592615
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: NODA KOUSUKE 北海道大学, 医学(系)研究科(研究院), 准教授 (90296666)
• Co-Investigator(Kenkyū-buntansha): KANDA Atsuhiro 北海道大学, 大学院医学研究科, 特任講師 (80342707)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 糖尿病網膜症 / 白血球接着分子 / 酸化ストレス / VAP-1 / MMPs

Outline of Final Research Achievements

Vascular adhesion protein (VAP)-1, a multifunctional molecule with adhesive and enzymatic properties, is expressed at the surface of vascular endothelial cells of mammals. It also exists as a soluble form (sVAP-1), which is implicated in oxidative stress via its enzymatic activity. This study explores a mechanistic components to form sVAP-1 in retinal endothelial cells. Retinal capillary endothelial cells released sVAP-1 when stimulated with high glucose or inflammatory cytokines such as tumor necrosis factor-α , interleukin-1β and vascular endothelial growth factor in vitro. Furthermore, matrix metalloproteinase -2 and -9, type IV collagenases, were the key molecules to mediate the protein cleavage of VAP-1 from retinal capillary endothelial cells. Our data for the first time provide the evidence on the sVAP-1 production mediated by inflammatory cytokines and type IV collagenases in the pathogenesis of diabetic retinopathy.

Free Research Field

糖尿病網膜症