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2014 Fiscal Year Final Research Report

Establishment of lacrimal gland epithelial cell line for the evaluation of lacrimal gland cells

Research Project

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Project/Area Number 24592650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionKeio University

Principal Investigator

KAWAKITA TETSUYA  慶應義塾大学, 医学部, 講師 (50408308)

Research Collaborator RYU In  慶應義塾大学, 医学部
IKEURA Kazuhiro  慶應義塾大学, 医学部
SOMA Yoshio  慶應義塾大学, 医学部, 准教授 (60268183)
Project Period (FY) 2012-04-01 – 2015-03-31
Keywords涙腺 / 細胞株
Outline of Final Research Achievements

We established lacrimal gland epithelial cell line spontaneously from 7-week old CD1 mice. Cyst-like cell aggregates were generated with modifying condition of cultivated time, culture medium, and enzyme treating time. using the defined conditions form established cell line and primary culture. Those cyst were used to observe and analyze the water movement by Coherent Anti-Stokes Raman Scattering microscope. Although water movement could be observed, we need to generate cysts with the same size morphology for applying assay as the function of lacrimal glands. We need further study to solve this problems.

Free Research Field

細胞生物学

URL: 

Published: 2016-06-03  

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