2014 Fiscal Year Final Research Report
The effect of Mast cell Tryptase to the Expression of Eotaxin-1/CCL11, Eotaxin-2/CCL24 and Eotaxin-3/CCL26 by Conjunctival Fibroblasts.
| Project/Area Number |
24592653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Akira 順天堂大学, 医学部, 准教授 (00312348)
FUNAKI Toshinari 順天堂大学, 医学部, 准教授 (80384121)
MURAKAMI Akira 順天堂大学, 医学部, 教授 (90157743)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 痒み（itch） / トリプターゼ（tryptase） / エオタキシン（eotaxin） / 結膜線維芽細胞 / 春季カタル / Nc/Ngaマウス |
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| Outline of Final Research Achievements |
To investigate the effect of mast cell tryptase to the expression of eotaxin-1, eotaxin-2 and eotaxin-3 by conjunctival fibroblasts. The production of eotaxin-1, -2 and -3 by conjunctival fibroblasts stimulated with or without IL-4/IL-13 or/and TGF-β1 was estimated by ELISA and PCR. Conjunctival fibroblasts produced eotaxin-1 and -3, but not eotaxin-2. Stimulation with IL-4/IL-13 and TGF-β1 synergistically increased eotaxin-1 and -3 productions. Tryptase reduced the immunoreactivity of eotaxin-1 and -3, but not eotaxin-2, due to the proteolysis of these eotaxins, but not the inhibition of its m-RNA expression. Mast cell tryptase decreases the eotaxin-1 and -3 produced by conjunctival fibroblasts, resulting the inhibition for the excessive accumulation of eosinophils in the conjunctiva. Therefore, eotaxin-2 in the tear may be a biomarker of severity of allergic conjunctival disease.
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| Free Research Field |
角結膜疾患、眼免疫学、眼アレルギー学
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