Development of the method for ex vivo expansion of corneal epithelial cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 24592675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Osaka University (2013-2015); Kyoto Prefectural University of Medicine (2012)
• Principal Investigator: KAWASAKI Satoshi 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (60347458)
• Co-Investigator(Kenkyū-buntansha): UENO Morio 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40426531)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 再生医療 / 膠様滴状角膜ジストロフィ / レチノブラストーマ / 細胞老化 / 創薬

Outline of Final Research Achievements

In regenerative medicine, efficient cell proliferation is a very important factor. In the present study, we focused on constructing cells for the screening of small-molecule drugs that enhance cell proliferation. Cells were constructed to express the well-known tumor-suppressor retinoblastoma (pRb) protein by introducing the RB1 gene that is regulated under the control of tetracycline to an osteosarcoma cell line. As expected, when pRb protein was expressed in the constructed cell by adding tetracycline, cell proliferation arrest and cell senescence was induced in the cells. If cell proliferation can be observed when simultaneously adding tetracycline and certain small-molecule drugs to the cells, the small-molecule drug may have an inhibitory effect on the pRb protein in terms of the arrest of cell proliferation. Among the 48 clones that we obtained, the best clone achieved 0.8 in Z’ value. The cells may be useful for the screening of small-molecule drugs to inhibit pRb protein.

Free Research Field

再生医療、創薬、分子生物学