Study of horizontal gene transfer and recombination mechanism in the anginosus streptococci

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592782
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: TAKAO Ayuko 鶴見大学, 歯学部, 学内講師 (10163156)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アンギノーサスレンサ球菌 / クオラムセンシング / ペプチドフェロモン / 遺伝子組換え / バクテリオシン / バイオフィルム形成

Outline of Final Research Achievements

The gene systems of com and blp regulate DNA recombination induced by competence-stimulating peptide (CSP) and bacteriocin production signaled by bacteriocin-inducing peptide (BIP), respectively. To know the mechanisms of horizontal gene transfer and natural recombination in Streptococcus intermedius, efficiency of transformation, efficacies of bacteriocin production and of　biofilm formation were investigated using the gene deletion mutants.
The experimental results suggested that the systems of com and blp regulated negatively with each other. Statistical analysis showed that bacteriocin production on the plate cultured bacteria and extracellular DNA amount in the biofilm culture significantly correlated. Therefore, bacteriocin might positively affect to horizontal gene transfer by its bactericidal effect to the genetically related bacteria, while its production was suppressed by the competence-related gene regulation system.

Free Research Field

口腔微生物学