The role of the initial colonizer Actinomyces oris in dental plaque formation.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24592790
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: MASHIMO Chiho 大阪歯科大学, 歯学部, 講師 (80368159)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 口腔細菌 / バイオフィルム / アクチノマイセス属細菌 / 初期付着菌

Outline of Final Research Achievements

Recent studies have revealed that oral Actinomyces spp. plays important roles for oral multispecies biofilm development as an initial colonizer. To identify gene(s) responsible for the extracellular polymeric substances (EPS) production, transposon mutagenesis was performed against a clinical isolate of A. oris strain K20. We reported the identification of genes that were predicted to be involved in the EPS biosynthesis and were essential for biofilm formation. We also developed the genetic engineering systems in Actinomyces spp. based on pJRD215 with high transformation efficiency.

Free Research Field

医歯薬学