Visualization of immune-inhibitory signaling in memory formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592828
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: University of Occupational and Environmental Health, Japan (2013-2014); Tokyo Medical and Dental University (2012)
• Principal Investigator: IWAI Yoshiko 産業医科大学, 医学部, 教授 (90362467)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Ｔ細胞 / 樹状細胞

Outline of Final Research Achievements

To visualize BATF expression during memory T cell formation, reporter gene constructs were generated by fusing the luciferase, GFP, or dsRED gene to the BATF promoter. Interestingly, BATF expression was induced both in T cells and dendritic cells upon stimulation. While BATF expression was induced upon TLR stimulation in murine dendritic cells derived from spleen and bone marrow, BATF was constitutively expressed in dendritic cell lines, suggesting that mechanisms regulating BATF expression in primary cells and cell lines may be different. These results suggest that it might be important to establish the reporter system using primary cells for the analysis of molecular mechanisms regulating BATF-expression.

Free Research Field

免疫学