Elucidation of mechanism on dentin hypersensitivity; cell response by hypertonic stress.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592876
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Kagoshima University
• Principal Investigator: MASAYUKI Tokuda 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (20253891)
• Co-Investigator(Kenkyū-buntansha): MORIMOTO Yoko 鹿児島大学, 医学部・歯学部附属病院, 助教 (30437967)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 象牙質知覚過敏症

Outline of Final Research Achievements

Osmotic stress is one cause of dental pain due to caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress. Cell viability decreased over 700 mOsm for 3 h cell culture. The shapes of cells and nuclei became irregular and vacuolar under hyperosmotic stress. The expression of cleaved caspase-3 was increased after treatment with hyperosmotic stress. Flow cytometry analysis detected no annexin V-positive cells, indicating apoptosis. Three MAPKs phosphorylation were induced by hyperosmotic stress. Inhibitors of three MAPKs inhibited the hyperosmotic stress-induced decline in cell cultures at 500 and 700 mOsm. Hyperosmotic stress induces cell death of OLCs with sucrose through a MAPK pathway.

Free Research Field

歯科保存学