Effect of anti-oxidant agent on TMJ chondrocytes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592903
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: UENO Takeshi 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (30359674)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 酸化ストレス / 抗酸化能

Outline of Final Research Achievements

Dental treatment often requires management of inflammation. The inflammation is closely associated with oxidative stress which hinders and delays the healing process. However, therapeutic approach focused on controlling oxidative stress in dental treatment is seldom found. This study tested the protective potential of an anti-oxidant, amino-acid derivative (AAD), in controlling the oxidative stress against TMJ chondrocytes. The inflammatory codition was simulated by adding hydrogen peroxide (H2O2) into culture medium. The exposure of H2O2 decreased the number of the cells, and both aggrecan and collagen II gene expressions. AAD addition into the H2O2 cultures restored the number of the cells, and the gene expression and glycosaminoglycan deposition to the level equivalent to untreated control culture. However, more importantly, the addition of AAD into the damaged culture restores their proliferative and differentiation capacities even under oxidative stress.

Free Research Field

歯科補綴学