2014 Fiscal Year Final Research Report
Screening of taste dysfunction using zinc-binding protein in saliva
Project/Area Number |
24592928
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Prosthetic dentistry
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Research Institution | Iwate Medical University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 味覚 / 亜鉛欠乏 / 炭酸脱水酵素 / イムノクロマト法 / 耳下腺唾液 / 味覚障害 / 体外診断薬 |
Outline of Final Research Achievements |
It is known that over 60% of patients with taste dysfunction are originally caused by zinc deficiency. Carbonic anhydrase (CA)Ⅵ, identical to gustin as a zinc-binding metalloprotein in human saliva, has significant relation to taste dysfunction. We have developed a swift and simple new diagnostic method using the immunochromatographic analysis. In this experiment, we examined a relationship between CA6 value and serum zinc level by using parotid saliva of patients with taste dysfunction, measured by the immunochromatographic method. We recognized a slight positive correlation between immunochromatographic measurement value. Furthermore, we attempted to apply the monoclonal antibody. Only LS-C196794 monoclonal antibody indicated comparable chromogenic reaction to polyclonal antibody we previously presented. Those results suggest that the measurement of zinc-binding protein in parotid saliva might be helpful to establish newly rapid diagnosis of zinc deficiency taste dysfunction.
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Free Research Field |
味覚障害
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