Detection of oral cancer stem cell which indicates epithelial to mesenchymal transition and development of new cancer treatment that targeted oral cancer stem cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592979
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Akita University
• Principal Investigator: NAKATA AKIRA 秋田大学, 医学部, 助教 (50400510)
• Co-Investigator(Kenkyū-buntansha): FUKUDA Masayuki 秋田大学, 医学部, 准教授 (20272049) ; SUGUYAMA Toshihiro 秋田大学, 医学部, 定年退職 (00127242)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 口腔癌幹細胞 / Snail / 上皮間葉形質転換 / 浸潤 / 転移 / 免疫抑制

Outline of Final Research Achievements

In this study, we try to detect oral cancer stem cell and clear that a part of cancer cell which gain invasion ability, express "Snail" molecule, indicates epithelial to mesenchymal transition and changes into the favorable character of invasion and metastasis.
First, cDNA of human Snail was included in expression vector for culture cell of mammals and gene was introduced into tongue cancer cell line (HSC4). when Snail molecule was introduced into HSC4 cells that was cobblestone-shaped form, which changes into the spindle-shaped form, and atttached weakly between cell to cell.Next, cultured HSC4 and HSC4/Snail cells were transplanted under the back of nude mice. For 3 months of transplantation and the size of the formed tumor were estimated. Tumorogenesis similar to human squamous cell carcinoma which is the history of HSC4 was seen, and Snail participated in promotion of tumorogenesis.

Free Research Field

口腔外科