2014 Fiscal Year Final Research Report
The isolation and identification of the higher bone differentiation of mesenchymal stem cell from the pulp of human teeth and the application for the jaw bone regeneration.
| Project/Area Number |
24592980
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHNEDA Osamu 筑波大学, 医学医療系, 教授 (30311872)
NAGANO Masumi 筑波大学, 医学医療系, 講師 (30436282)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 間葉系幹細胞 / 細胞表面マーカー / 自家移植 / 骨分化能 |
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| Outline of Final Research Achievements |
In the crown completion stage of the dental pulp derived mesenchymal stem cells (DPSCs), the expression of the Runx2 have been increased significantly in the DPSCs of root formation stage and root completed stage from the previous time of bone differentiation induction. This result was considered due to the Runx2 stage abnormal specific expression for potential the suppression of bone differentiation. In the bone differentiation process of crown completed stage from DPSCs, expression of ALP were significantly lower form the other timing derived DPSCs. Therefore, the expression of Runx2 was analyzed for bone differentiation potential by the gene transfer of ALP to the DPSCs of crown completion. As a result, the normal bone differentiation induction was observed in the ALP gene transfer group. But it revealed that suppressing the expression of Runx2 is not observed. In the future, we will analyze a system for regulation of expression of Runx2 in DPSC.
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| Free Research Field |
再生医学による顎骨再建
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