Development of tooth regenerative therapy using the interaction between immortalized odontogenic cell and iPS cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592989
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NAGAI Hirokazu 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (50282190)
• Co-Investigator(Kenkyū-buntansha): MIYAMOTO Youji 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (20200214) ; TAMATANI Tetsuya 徳島大学, 病院, 講師 (30274236) ; UCHIDA Daisuke 獨協医科大学, 医学部, 准教授 (20335798) ; OHE Go 徳島大学, 大学病院, 助教 (60432762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 再生医学 / 歯の再生 / iPS細胞

Outline of Final Research Achievements

In this study, we try to develop the tooth regenerative therapy using the interaction between immortalized odontogenic cell and human induced pluripotent stem (iPS) cells. We used iPS cells for cell source of tooth regenerative therapy, and used low crystalline carbonate apatite for scaffold of tooth regenerative therapy. As a result, it was revealed that 1) the differentiation of human iPS cells to mesenchymal stem cell-like cells was induced, 2) carbonate apatite was resorbed by osteoclast in vivo, 3) mesenchymal stem cells from iPS cells cultured on carbonate apatite disk differentiated into osteoblastic cells, 4) carbonate apatite granules combined with BMP-2 stimulated the ectopic new bone formation by promoting osteoblastic differentiation of mesenchymal cells in the surrounding tissue.

Free Research Field

医歯薬学