2014 Fiscal Year Final Research Report
Novel therapeutic approach using beta-glucan based oligonucleotides delivery system for osteoporosis
| Project/Area Number |
24593000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 破骨細胞 / シゾフィラン / dectin-1 / ドラッグデリバリー / カードラン |
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| Outline of Final Research Achievements |
Several immune system cell surface receptors are reported to be associated with osteoclastogenesis. Dectin 1, a lectin receptor for β-glucan, is found predominantly on cells of the myeloid lineage. In this study we have developed a delivery system for oligonucleotides using schizophyllan (SPG), a polysaccharide that belongs to the β-glucan family. Application of complex consisting SPG and TNF receptor associated factor 6 (TRAF6) specific siRNA to osteoclast progenitor cells inhibited the osteoclast formation in vitro.
On the other hand, we also demonstrated that the dectin-1 agonist curdlan potentially inhibits osteoclast differentiation, especially nuclear factor of activated T cell cytoplasmic 1 (NFATc1) expression, and that Syk kinase plays a crucial role in the transcriptional pathways.
These data suggest that application of this new complex and curdlan administration could be potential candidates for the treatment of osteoclast-related diseases such as osteoporosis.
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| Free Research Field |
細胞生物学
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