Elucidation of mechanism in alteration of salivary protein secretion due to chronic stress and its clinical use for dry mouth patients

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Sagami Women's University (2013-2014); Kamakura Women's University (2012)
• Principal Investigator: YOSHINO Yoko 相模女子大学, 公私立大学の部局等, 准教授 (70298248)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Yoichi 鶴見大学, 歯学部, 講師 (90148057)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 慢性ストレス / 唾液タンパク / カリクレイン / 小胞体ストレス / GRP78/Bip / phospho-eIF2α

Outline of Final Research Achievements

The objective of the present study was to examine the mechanism of decrease in salivary protein and kallikrein under chronic stress. Male ICR mice at 13 weeks of age were divided into two groups; Phenylephrine was intramuscularly injected with 5 mg/kg twice a day for 5 days (PHE) and physiological saline in the same time schedule (CTL). The pilocarpine-stimulated whole saliva was collected and the parotid and submandibular glands were removed. Salivary protein, EGF concentration and kallikrein activity were significanly decreased in the PHE in comparison with CTL. The CCh-induced Ca2+ release was significantly reduced in PHE groups compared with that in CTL groups. The expression of ER chaperone, glucose-regulated protein 78 (GRP78/Bip) and translational attenuation, phospho-eIF2α was significantly increased in the PHE groups. The area of secretory granule was significatly poorly-stained and decreased in PHE groups in coparison with CTL in hematoxylin eosin stain.

Free Research Field

口腔科学