Exploitation of MICA gene polymorphism for development of personalized medicine in oral cancer patients

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: TANI RYOUJI 広島大学, 大学病院, 助教 (10291486)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Tetsuji 広島大学, 医歯薬保健学研究院, 教授 (00169153) ; TORATANI Shigeaki 広島大学, 医歯薬保健学研究院, 准教授 (90172220)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: MICA gene polymorphism / oral cancer / soluble MICA / personalized medicine

Outline of Final Research Achievements

In this study, we examined the expression of MICA proten in cell extracts using the Western Blot and the expression of MICA mRNA using Northern Blot in OSCC cell lines. We found expression of MICA mRNA in all OSCC cell lines, and expression of MICA proten in KO cell lines was lowest. The soluble MICA (sMICA) in conditioned medium was measured by ELISA and we found the high level of sMICA in KO cells. As we have purified sMICA produced by KO cells, the molecular weight of sMICA produced by KO cells was 24,000. Furthemore, TGF-β1 and TGF-β2 was concentration-dependent manner to promote the production of sMICA. Expression of cell surface MICA protein was enhanced and production of sMICA into the culture supernatant was significantly suppressed in KO cells transfected with TGF-β1/2 siRNA.

Free Research Field

口腔外科