Development of a novel functional preservation therapy against advanced, recurrent or unresectable oral cancers.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Yamaguchi University
• Principal Investigator: HARADA Koji 山口大学, 医学(系)研究科(研究院), 助教 (60253217)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 人工癌幹細胞 / リプログラミング因子 / エピソーマルベクター / 口腔癌

Outline of Final Research Achievements

It is often difficult to treat advanced, recurrent or unresectable oral cancers. A new strategy must be needed. In these years, the cancer stem cells (CSCs) are an important target for the development of novel strategies in above oral cancer treatment. However, CSCs-targeted new anti-cancer drug discovery is currently hindered by the lack of easy and reliable methods for collecting sufficient number of CSCs. Briefly,artificially-manufactured cancer stem cells are essential. In this project,we could generate the transfectant cells ( HSC2/hOCT3/4-shp53-F+hSK+hUL) with CSCs properties by introduction of defined reprogramming factors (Oct3/4, sh53, Sox2, Klf4, l-Myc and Lin28) into HSC2 tongue cancer cells. Our findings suggest that artificial cancer stem cells developed by cellular reprogramming may be useful for investigating the acquisition of potential malignancy as well as screening the CSCs-targeting drugs.

Free Research Field

歯科口腔外科学