Investigation of bacterial distribution in pregnant women by metagenomics analysis and prevention of adverse pregnancy outcomes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: KATO Satsuki 北海道医療大学, 歯学部, 講師 (50281283)
• Co-Investigator(Kenkyū-buntansha): MORI Mari 北海道医療大学, 歯学部, 講師 (30275490) ; NAGASAWA Toshiyuki 北海道医療大学, 歯学部, 教授 (90262203)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メタゲノム解析 / 歯周病 / 妊娠女性 / 早産・低体重児出産

Outline of Final Research Achievements

Previous studies have suggested that periodontal disease might be a risk factor for preterm birth and/or low birth weight. The aim of this study was to evaluate the association between oral bacteria and systemic conditions in pregnant women. Salivary samples were collected from pregnant women and non-pregnant women, and periodontal examination was performed. Concentrations of progesterone and estradiol were determined using ELISA. The bacterial DNA was isolated from saliva, and the bacterial composition of oral bacterium was investigated by 16S rRNA-based metagenomics analysis. It was found that progesterone levels in pregnant women were significantly higher than non-pregnant women. From 16S rRNA-based metagenomics analysis, Bifidobacteriales was only emerged in pregnant women. Periodontal status was not significantly different between pregnant women and non-pregnant women. These results suggest that Bifidobacteriales might be increased in pregnant women.

Free Research Field

歯周治療学