Elucidation of the alveolar bone absorption mechanism by the oral malodorous compounds .

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IMAI TOSHIO 日本歯科大学, 生命歯学部, 教授 (90120617)
• Co-Investigator(Kenkyū-buntansha): YAEGAKI Ken 日本歯科大学, 生命歯学部, 教授 (40166468) ; KAMODA Takeshi 日本歯科大学, 新潟生命歯学部, 講師 (00366767)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マウス骨髄幹細胞 / 破骨細胞 / 骨芽細胞 / 硫化水素 / 口臭

Outline of Final Research Achievements

TThis study was aimed to elucidate the effect of hydrogen sulfide on the differentiation mechanism of bone marrow stem cells. Bone marrow stem cells were isolated from mouse femur using stem cell separation device. CD114 (+) positive cells (BMC-CD117 (+)) were isolated from the stem cells using a Magnetic separation system . NFAT1 and Aggrecan Neoepitope were present in the BMC-CD117 (+). BMC-CD117 (+) were cultured for 3 days with hydrogen sulfide. Multinucleated cells of tartrate-resistant acid phosphatase-positive that is a differentiation marker of osteoclast were significantly observed. Hydrogen sulfide was induced differentiation of osteoclasts from bone marrow stem cells, suggesting that it is a risk factor for alveolar bone resorption.

Free Research Field

衛生学