Roles of plant extracts in therapeutic and preventive agents for periodontitis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: WATANABE Kiyoko 神奈川歯科大学, 歯学研究科(研究院), 講師 (70148021)
• Co-Investigator(Kenkyū-buntansha): HAMADA Nobushiro 神奈川歯科大学, 大学院歯学研究科, 教授 (20247315)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物由来ポリフェノール / 歯周炎 / 抗菌活性 / 歯槽骨吸収抑制効果 / 付着・定着阻害 / 口臭抑制 / 唾液細菌 / 歯周健康

Outline of Final Research Achievements

We examined the inhibitory effects of Pycnogenol (PYC) on alveolar bone resorption induced by P. gingivalis and osteoclast differentiation. In rat periodontitis model, rats were fed a regular diet or PYC mixed diet (0.025%: w/w). Administration of PYC along with P. gingivalis infection significantly reduced alveolar bone resorption. Treatment of P. gingivalis with 1 μg/ml PYC reduced the number of viable bacterial cells. Addition of PYC to epithelial cells inhibited adhesion and invasion by P. gingivalis. PYC treatment significantly inhibited osteoclast formation. Addition of PYC (100 μg/ml, 10 μg/ml or 1 μg/ml) to purified osteoclasts culture induced cell apoptosis. These results suggest that PYC may prevent alveolar bone resorption through its antibacterial activity against P. gingivalis and by suppressing osteoclastogenesis. Therefore, PYC may be useful as a therapeutic and preventative agent for bone diseases such as periodontitis.

Free Research Field

微生物学