Search on the biomarker for the identification of Cl2 and phosgene exposure

2014 Fiscal Year Final Research Report

• Project/Area Number: 24612004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Reliable environmental measurement methods
• Research Institution: National Research Institute of Police Science
• Principal Investigator: TSUGE Koichiro 科学警察研究所, 法科学第三部, 主任研究官 (90356204)
• Co-Investigator(Kenkyū-buntansha): OHMORI Takeshi 科学警察研究所, 室長 (70356195)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 塩素 / 法中毒 / 法医学 / 曝露証明 / プロテオーム

Outline of Final Research Achievements

The chloric gas and phosgene are used widely in the chemical industry field. There are also more possibilities that these are used by terrorism as a chemical weapon. However, determination method of exposure by these toxic gases is not established.
Model protein was exposed to a sodium hypochlorite and digested by the proteases.
The digested peptide mixture was injected to LC-MS/MS. To compare the peak area of exposed and non-exposed samples, a few peptides that peak area decreases significantly were found. LC-MS/MS analysis was carried out using the mass number which presumed chlorine to have combined. However, we did not determine the chlorine combined peptides.

Free Research Field

法医学　環境系薬学