Functional analysis of a placenta-specific imprinted gene Gab1 in the placenta

2014 Fiscal Year Final Research Report

• Project/Area Number: 24613001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: Tohoku University
• Principal Investigator: OKAE Hiroaki 東北大学, 医学(系)研究科(研究院), 助教 (10582695)
• Co-Investigator(Kenkyū-buntansha): ARIMA Takahiro 東北大学, 大学院医学系研究科, 教授 (80253532)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ゲノムインプリンティング / メチル化インプリント / 胎盤幹（TS）細胞

Outline of Final Research Achievements

Gab1 is a novel placenta-specific imprinted gene and we analyzed the function and regulatory mechanism of Gab1 using Gab1 KO mice, trophoblast stem cells and cloned mice. We found that the paternal expression of Gab1 was regulated by a novel differentially methylated region (DMR) and the imprinted expression was important for normal placental development. We also found that in cloned mouse placentas, the Gab1 DMR was lost and Gab1 was highly expressed, which may contribute to placentomegaly of cloned mice.

Free Research Field

分子生物学