2014 Fiscal Year Final Research Report
Spatial positioning of related genes during mouse reprograming for epigenetic regulation
| Project/Area Number |
24613003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
epigenetics
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
TANABE Hideyuki 総合研究大学院大学, 先導科学研究科, 准教授 (50261178)
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| Co-Investigator(Renkei-kenkyūsha) |
MITANI Tasuku 近畿大学, 先端科学技術研究所, 教授 (10322265)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 染色体テリトリー / 3D-FISH / 遺伝子空間配置 / 核内配置 / マウスiPS細胞 / リプログラミング / ゲノム配置 |
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| Outline of Final Research Achievements |
In this study from the viewpoint of spatial positioning of chromosome territories and genomic regions within the nucleus I would try to find the molecular mechanism of why the iPS cells are established and to investigate gene kissing of reprograming related genes by 3D-FISH techniques using the DNA probes with Oct3/4, Sox2, Klf4, c-Myc, and Nanog, and their chromosomal paints onto mice cell nuclei. Basic characteristics of spatial arrangements have not yet been carried out due to shortage of available cells, however, when overcoming technical problems, I would perform the analyses and discuss the issues.
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| Free Research Field |
分子細胞遺伝学
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