2015 Fiscal Year Final Research Report
Mechanism of neural stem cell maintenance by chromatin regulator
| Project/Area Number |
24613006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
epigenetics
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | エピジェネティクス / クロマチン / 神経幹細胞 / ヒストンアセチル化 / 細胞増殖 / 遺伝子発現 / マウスモデル |
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| Outline of Final Research Achievements |
Epigenetic mechanism via chromatin regulation is crucial for many aspects of biological processes including maintenance of stem cells. MRG15 is the chromodomain-containing and chromatin-binding protein. It is known that MRG15 involves in gene regulation and splicing via making large complexes with other proteins. To investigate the role of MRG15 in neural stem/progenitor cells, we have produced MRG15 deficient mice in which MRG15 was specifically deleted in neural stem/progenitor cells. Although these mice were born in accordance with Mendelian ratio, their body size as well as brain weight was significantly reduced compared with that of control littermates. Moreover, in vitro growth ability of the neural stem/progenitor cells isolated from these mice was strongly suppressed. These results suggest that gene regulation and splicing in which MRG15 involves have a pivotal role in maintenance and function of neural stem/progenitor cells.
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| Free Research Field |
細胞生物学
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