2014 Fiscal Year Final Research Report
Regulation of protein synthesis and degradation in skeletal muscle by amino acids which are not used for protein synthesis
| Project/Area Number |
24614002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | Iwate University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 骨格筋萎縮 / タンパク質合成 / タンパク質分解 / シトルリン / リジン / サッカロピン |
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| Outline of Final Research Achievements |
The mechanisms of muscle protein synthesis and degradation by citrulline which is not used for protein synthesis were elucidated using C2C12 myotube cells. C2C12 cells treated with citrulline showed suppression of protein degradation through autophagy/lysosome proteolytic system. It is suggested that this suppression was regulated by mTOR. Intracellular arginine concentration was not increased by citrulline treatment, therefore the effects of citrulline may due to citrulline itself.
Saccharopine, a metabolite of lysine, suppressed protein degradation in C2C12 myotube cells by reduction of autophagy/lysosome proteolytic system. This suppression is considered to be regulated by Akt/mTOR.
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| Free Research Field |
栄養化学
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