Proteomic analysis on development and differentiation of human neural stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24619001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Chiba University
• Principal Investigator: AKAMA Kuniko 千葉大学, 普遍教育センター, 教授 (50114228)
• Co-Investigator(Kenkyū-buntansha): INOUE Nobuo 首都大学東京, 人間健康科学研究科, 客員教授 (50159985) ; NAKAYAMA Takashi 横浜市立大学, 医学部, 准教授 (90150060)
• Co-Investigator(Renkei-kenkyūsha): HIRANO Hisashi 横浜市立大学, 大学院生命ナノシステム科学研究科, 教授 (00275075)
• Research Collaborator: TOMIOKA Tetsutaro ; SHIBASAKI Gen ; TOTSUKA Akihiro ; KANO Yoshihiro
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞外マトリックス / 受容体 / シグナル伝達 / 細胞骨格制御 / 神経幹細胞の増殖 / 細胞伸長

Outline of Final Research Achievements

We investigated the differentially expressed proteins during differentiation of highly homogeneous human embryonic stem cells to early and late neural stem cells by neural stem sphere method, using 2-dimensional- and SDS-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry.
The results of classification of protein functions and search of metabolic pathways related to the differentially expressed proteins involved in these three differentiation stages suggested that ES cells differentiated to early neural stem cells via extracellular matrix-receptor interactions followed by their signal transduction and reorganization of cytoskeleton, and that early neural stem cells differentiated to late neural stem cells via reorganization of cytoskeleton followed by extension of the cells with increase of the differentiated neural stem cells.

Free Research Field

タンパク質化学