On-site diagnosis of periodontal disease by the multi-turn TOF mass spectrometer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24619002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Osaka University
• Principal Investigator: TOYODA Michisato 大阪大学, 理学(系)研究科(研究院), 教授 (80283828)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Shinya 大阪大学, 大学院歯学研究科, 教授 (70239490) ; BAMBA Takeshi 九州大学, 生体防御医学研究所, 教授 (10432444) ; SHIMMA Shuichi 国立がん研究センター, 研究員 (30515896)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メタボロミクス / 歯周病診断 / オンサイトマススペクトロメトリー

Outline of Final Research Achievements

The aim of this study is to examine the usefulness of gas chromatography/mass spectrometry (GC/MS) in metabolomic analysis of gingival crevicular fluid (GCF) in order to objectively diagnose periodontitis at a molecular level.
Nineteen metabolites were identified using GC/MS. Total ion current chromatograms showed broad differences in metabolite peak patterns between GCF samples obtained from healthy sites, moderate-pocket sites, and deep-pocket sites. The intensity difference of some metabolites was significant at sites with deep pockets compared to healthy sites. Principal component analysis confirmed these observations by clearly delineating healthy sites and sites with deep pockets. These results suggest that metabolomic analysis of GCF is useful for discovery of potential biomarkers in establishing a new diagnostic method for periodontitis.

Free Research Field

質量分析学