Development of low polar molecules-targetted Live single-cell MS by piezo-oscillated nano-CI.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24619004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Fukushima Medical University
• Principal Investigator: TSUYAMA NAOHIRO 福島県立医科大学, 医学部, 准教授 (10335747)
• Co-Investigator(Kenkyū-buntansha): MIZUNO HAJIME 理化学研究所, 生命システム研究センター, 研究員 (30457288) ; MASUJIMA TSUTOMU 理化学研究所, 生命システム研究センター, グループリーダー (10136054)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 質量分析 / メタボロミクス / ナノスプレー / 化学イオン化 / 一細胞分析

Outline of Final Research Achievements

We developed Live single-cell MS method which allows serial analysis from single cell content recovery to direct ionization of included molecules extracted with ionization solvent using a nanospray chip followed by mass spectrometric measurement. In order to apply this method for low-polar molecules, we made a nanospray-oscillation device using a piezo element to nebulize solvent. Ionization was achieved by a corona discharge device. We found that signal of low-polar molecules was increased whereas background signal of environmental origin was also increased. To improve background noise, we made a device for ionization in clean charcoal-treated nitrogen gas-environment. We confirmed that signals of low-polar molecules were increased compared to the original method.

Free Research Field

分析化学・放射線生物学