2014 Fiscal Year Final Research Report
Liquid extraction surface analysis of lipids from local tissue areas by mass spectrometry for pathological analysis
| Project/Area Number |
24619007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Chubu University |
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Principal Investigator |
TAGUCHI Ryo 中部大学, 応用生物学部, 客員教授 (20080210)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | メタボロミクス / リピドミクス / 質量分析 |
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| Outline of Final Research Achievements |
We applied a liquid extraction surface analysis (LESA) combination with NanoMate as a new approach for localization analysis of lipid molecular species in mammalian tissue slices and body fluid on segmented membrane. As results mass profiles of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in different areas of several tissue slices were effectively obtained by direct extraction. The profiles in molecular species of glycosphingolipids (gangliosides and sulfatides) and triglycerides were also effectively obtained by this method.
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| Free Research Field |
脂質生化学
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