Development of highly accurate and sensitive quantification method for protein and peptide in biological matrix

2014 Fiscal Year Final Research Report

• Project/Area Number: 24619012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KINUMI Tomoya 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (90293125)
• Co-Investigator(Kenkyū-buntansha): NAKAJIMA Yoshihiro 産業技術総合研究所, 健康工学研究部門, グループ長 (10291080)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 質量分析 / 同位体希釈 / タンパク質 / ペプチド / 血清 / 定量分析 / 化学修飾

Outline of Final Research Achievements

Highly accurate and sensitive quantification of serum protein and peptide by mass spectrometry is a key technology which provides the standard value to calibrate and compare the results in clinical chemistry and other research field related to quantification. We performed amino acid analyses of pure C-peptide and C reactive protein with high accuracy, and established certified reference materials of these proteins. To achieve highly accurate and sensitive quantification for serum C-peptide and C reactive protein, we also developed the quantification methods utilizing purification and N-terminal modification methods to enhance the signal intensity.

Free Research Field

分析化学・質量分析学・タンパク質化学