Study of space radiation using ES cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24620007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Osaka City University
• Principal Investigator: MORITA Takashi 大阪市立大学, 医学(系)研究科(研究院), 教授 (70150349)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Kayo 大阪市立大学, 医学(系)研究科(研究院), 准教授 (30311921) ; KIZU Akane 大阪市立大学, 医学(系)研究科(研究院), 助教 (30623201)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 宇宙放射線 / ES細胞 / 染色体異常

Outline of Final Research Achievements

It is important to estimate the influence of space radiation on the human body during long stays in space including missions to Internatonal Space Station (ISS), the Earth’s moon, or Mars. In order to protect space radiation, we investigate the effect of stimulation of DNA repair genes, such as histone H2AX. We made plasmid vector using Tet-on inducing system to produce histone H2AX. The vector was introduced in ES cells and H2AX gene was induced to express by Doxycyline addition. It resulted in up-regulation of H2AX gene expression and increased number of foci of γ-H2AX foci after irradiation.

Free Research Field

分子生物学