Investigating potential relationship between circadian clodk and skeletal muscle atrophy.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24620015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: NAKAO Reiko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (20582696)
• Co-Investigator(Renkei-kenkyūsha): OISHI Katsutaka 国立研究開発法人 産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (50338688) ; MIYAZAKI Koyomi 国立研究開発法人 産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (70358125) ; SHIMBA Shigeki 日本大学, 薬学部, 教授 (20287668)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 筋萎縮 / 概日リズム / 時計遺伝子 / 末梢時計

Outline of Final Research Achievements

We analyzed the effects of unilateral sciatic denervation on the expression of circadian clock genes in the gastrocnemius muscles of mice. Expression levels of most clock genes such as Bmal1, Per1, Rora, Nr1d1 and Dbp were reduced in accordance with the extent of muscle atrophy, although circadian Per2 expression was significantly augmented. The circadian expression of Bmal1 and Dbp was phase advanced in denervated muscle. The mRNA expression of Clock was significantly increased in denervated muscle and it was not affected by atrophic progression. Sciatic denervation did not affect the expression of these genes in the contralateral muscle, suggesting that humoral changes were not involved in denervation-induced muscle clock disruption. Our findings revealed that sciatic denervation disrupts the circadian expression of clock genes either directly or indirectly via muscle atrophy in the gastrocnemius muscles of mice in a gene-specific manner.

Free Research Field

栄養学、時間生物学