Quantitative understanding for neural plasticity of C. elegans with newly developed micro-fluidic device

2013 Fiscal Year Final Research Report

• Project/Area Number: 24650159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Bioinformatics/Life informatics
• Research Institution: Keio University
• Principal Investigator: OKA KOTARO 慶應義塾大学, 理工学部, 教授 (10276412)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 線虫 / 神経回路 / 特定神経細胞破壊 / 細胞膜電位計測 / 細胞カルシウム計測 / マイクロ流体デバイス

Research Abstract

Caenorhabditis elegans is known as one of model animals, and it has been used for understanding the relationship between neural functions and behaviors. The worm senses odors and shows taxis toward the source of favorite odor by detecting its concentration gradient. However, little is known how exactly worms can discriminate the concentration of odor and change their head turning behavior in regard to the response of olfactory neurons. For demonstrating the role of single neurons in specific behaviors, we investigated the neural activity from a single neuron by Ca and membrane potential imaging, and also quantitative behaviors by developing new types of micro-fluidic devices. We succeeded to visualize the Ca oscillation synchronized with the head movement during odor stimulation in AIY interneuron.

Research Products

• [Journal Article] A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed (2013)
  • Author(s): Kobayashi J, Shidara H, Morisawa Y, Kawakami M, Tanahashi Y, Hotta K, Oka K
  • Journal Title: Neurosci Lett Volume: 548 Pages: 261-264
  • DOI: 10.1016/j.neulet.2013.05.053
  • Peer Reviewed
• [Journal Article] Odorant-induced membrane potential depolarization of AIY interneuron in Caenorhabditis elegans (2013)
  • Author(s): Shidara H, Kobayashi J, Tanamoto R, Hotta K, Oka K
  • Journal Title: Neurosci Lett Volume: 541 Pages: 199-203
  • DOI: 10.1016/j.neulet.2013.02.016
  • Peer Reviewed
• [Presentation] AIY interneurons involved in the cross modality adaptation in Caenorhabditis elegans (2013)
  • Author(s): Shidara H, Kobayashi J, Tanamoto R, Hotta K, Oka K
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-11
• [Presentation] Response to olfaction in Caenorhabditis elegans follows the Weber-Fechner law (2013)
  • Author(s): Makino M, Shidara H, Hotta K, Oka K
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-11
• [Presentation] Sensory stimulation from a specific modality adapts a different modality in Caenorhabditis elegans (2013)
  • Author(s): Shidara H, Kobayashi J, Tanamoro R, Hotta K, Oka K
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館, 京都
  • Year and Date: 2013-10-30
• [Presentation] 線虫において異なる感覚情報が相互に与える影響 (2013)
  • Author(s): 設樂久志, 小林純也, 棚元亮, 堀田耕司, 岡浩太郎
  • Organizer: 第22回日本バイオイメージング学会学術集会
  • Place of Presentation: 東京大学薬学部講堂, 東京
  • Year and Date: 2013-09-16
• [Presentation] 線虫における交差順応 (2013)
  • Author(s): 設樂久志, 小林純也, 棚元亮, 堀田耕司, 岡浩太郎
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館, 京都
  • Year and Date: 2013-06-21
• [Presentation] Neural activity visualization of AIY interneuron in Caenorhabditis elegans with voltage- sensitive fluorescent protein (2012)
  • Author(s): Shidara H, Kobayashi J, Tanamoto R, Hotta K, Oka K
  • Organizer: Society for Neuroscience 2012
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-14
• [Presentation] 線虫AIY介在神経細胞での局在した神経活動 : 蛍光イメージングによる研究 (2012)
  • Author(s): 設樂久志, 小林純也, 棚元亮, 堀田耕司, 岡浩太郎
  • Organizer: 第50回日本生物物理学会
  • Place of Presentation: 名古屋大学東山キャンパス, 名古屋
  • Year and Date: 2012-09-24
• [Presentation] 線虫介在神経細胞AIYの膜電位イメージング (2012)
  • Author(s): 設樂久志, 小林純也, 棚元亮, 堀田耕司, 岡浩太郎
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場, 名古屋
  • Year and Date: 2012-09-20