2013 Fiscal Year Final Research Report
Analysis of neural plasticity and behavioral diversity by the inducible method of single neuron gene expression
| Project/Area Number |
24650165
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Nagoya University |
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Principal Investigator |
TANIMOTO Masashi 名古屋大学, 理学(系)研究科(研究院), 助教 (30608716)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Yoichi 名古屋大学, 大学院理学研究科, 助教 (00144444)
TAKAGI Shin 名古屋大学, 大学院理学研究科, 准教授 (90171420)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 遺伝子発現誘導 / 神経細胞 / 近赤外レーザー / 光ファイバ |
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| Research Abstract |
Behavioral analysis on sound/vibration-evoked fast escapes in larval zebrafish showed that Mauthner (M-) cell played a crucial role in the initiation of the escapes. In the absence of the M-cell, non-M circuits could trigger the fast escapes to the left or right, while larvae sometimes exhibited aberrant escapes. By using transgenic lines that drive heat-inducible expression of green fluorescent protein (GFP), edge-processed optical fibers and infrared laser, we succeeded in inducing GFP in targeted single or multiple neurons in embryonic and larval zebrafish brain.
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