2013 Fiscal Year Final Research Report
A new multitasking gene specifically developed for the analysis of brain functions
| Project/Area Number |
24650195
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 遺伝子 / ノックアウトマウス / Cre/loxP / Flp/frt / 脳機能解析 |
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| Research Abstract |
In the analysis of brain functions using conditional knockout mice, the ability of Cre driver mice is important and determines the quality of research. The purpose of this study is to produce a new multitasking cassette which can express a specific gene in a cell-specific manner without affecting the endogenous gene expression. When a multitasking cassette is inserted into a specific driver gene, its driver gene is knocked out. Next, the stop sequences are removed, Cre can be activated under the corresponding promoter with the endogenous gene expression kept intact. Also, the corresponding gene expression can be rescued by the Flpase. After checking the function of this cassette in cultured cells, we next produced a knockin mouse with the cassette integrated in the Abat gene. The generated Abat-knockin mice and their Cre activity is being checked using the lacZ reporter mice. This system can contribute in generating driver mice with only one line, for the use of many purposes.
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