Long-lasting synaptic plasticity subjected to the stochastic principle

2013 Fiscal Year Final Research Report

• Project/Area Number: 24650207
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Osaka University
• Principal Investigator: OGURA Akihiko 大阪大学, 生命機能研究科, 教授 (30260631)
• Co-Investigator(Kenkyū-buntansha): TOMINAGA Keiko (YOSHINO Keiko) 大阪大学, 大学院生命機能研究科, 准教授 (60256196)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: シナプス可塑性 / シナプス新生 / シナプス廃止 / ゆらぎ / 脳切片培養

Research Abstract

We have proposed that 2 novel synaptic plasticity phenomena in the stable cultures of hippocampal slice should serve as suitable models for the analyses of cellular mechanisms underlying memory consolidation. Those are RISE (long-lasting synaptic enhancement after 3 repeated inductions of LTP supported by new synapse formation) and LOSS (long-lasting synaptic weakening after 3 repeated inductions of LTD supported by synapse elimination). In the present study we demonstrated that RISE develops through the following phases: a) the synapses are under constant generation and retraction; b) both the rates of generation and retraction increase transiently after a RISE-producing stimulus, c) the retraction rate returns to the basal level earlier than the generation rate, leading to a net increase in synapse density. However, LOSS develops without the phase of b. The 2 apparently symmetric phenomena are in fact asymmetric in the process of development.

Research Products

• [Journal Article] 記憶固定の細胞生物学 (2014)
  • Author(s): 小倉明彦,冨永恵子
  • Journal Title: 科学 Volume: 83 Pages: 256-262
• [Journal Article] Dendritic spine dynamics in synaptogenesis after repeated LTP inductions : Dependence on pre-existing spine density (2013)
  • Author(s): Oe, Y., Tominaga-Yoshino, K., Hasegawa, S. & Ogura, A.
  • Journal Title: Sci. Rep Volume: 3(on line publication) Pages: 1957
  • DOI: 10.1038/srep01957
  • Peer Reviewed
• [Journal Article] Involvement of TrkB- and p75^<NTR>-signaling pathways in two contrasting forms of long-lasting synaptic plasticity (2013)
  • Author(s): Sakuragi, S., Tominaga-Yoshino, K. & Ogura, A.
  • Journal Title: Sci. Rep Volume: 3(on line publication) Pages: 3158
  • DOI: 10.1038/srep03158
  • Peer Reviewed
• [Journal Article] 長期記憶の細胞機構解明を目指して (2013)
  • Author(s): 冨永恵子,小倉明彦
  • Journal Title: 日薬理誌 Volume: 142 Pages: 122-127
  • Peer Reviewed
• [Journal Article] A simplified method to generate serotonergic neurons from mouse embryonic stem and induced pluripotent stem cells (2012)
  • Author(s): Shimada, T.,Takai, Y., Shinohara, K., Yamasaki, A., Tominaga-Yoshino, K., Ogura, A., Toi, A., Asano, K., Shintani, N., Hayata-Takano, A., Baba, A. & Hashimoto, H.
  • Journal Title: J. Neurochem Volume: 122 Pages: 81-93
  • Peer Reviewed
• [Presentation] RISE(繰り返しLTP誘発後の長期シナプス強化)の生理的意義 : 個体での検討 (2014)
  • Author(s): 永岡昭吾、浦久保知佳、冨永-吉野恵子、小倉明彦
  • Organizer: 日本生理学会
  • Place of Presentation: (鹿児島)
  • Year and Date: 20140300
• [Presentation] 繰り返しLTD誘発後の長期持続的シナプス減弱(LOSS)生起時の樹状突起棘の動態 (2013)
  • Author(s): 長谷川翔、冨永-吉野恵子、小倉明彦
  • Organizer: 生理研研究会
  • Place of Presentation: (岡崎)
  • Year and Date: 20131200
• [Presentation] Asymmetric dendritic spine dynamics in the apparently symmetric long-lasting synaptic plasticity phenomena after repeated LTP/LTD inductions (2013)
  • Author(s): Hasegawa, S., Oe, Y., Tominaga-Yoshino, K., Ogura, A.
  • Organizer: Society for Neuroscience
  • Place of Presentation: (San Diego)
  • Year and Date: 20131100
• [Presentation] BDNFとその前駆体分子の作用による対照的な長期可塑性 (2013)
  • Author(s): 桜木繁雄、冨永-吉野恵子、小倉明彦
  • Organizer: 日本神経科学学会・日本神経化学会合同大会
  • Place of Presentation: (京都)
  • Year and Date: 20130600
• [Presentation] 繰り返しLTD誘発後の長期持続性シナプス減弱での海馬錐体細胞樹状突起棘の動態 (2013)
  • Author(s): 長谷川翔、大江祐樹、冨永-吉野恵子、小倉明彦
  • Organizer: 日本神経科学学会・日本神経化学会合同大会
  • Place of Presentation: (京都)
  • Year and Date: 20130600
• [Presentation] 苔状線維CA3間シナプスで繰り返し長期増強の誘発後に生起する可塑的変化 (2013)
  • Author(s): 有賀理瑛、冨永-吉野恵子、小倉明彦
  • Organizer: 日本神経科学学会・日本神経化学会合同大会
  • Place of Presentation: (京都)
  • Year and Date: 20130600
• [Presentation] 繰り返しLTD誘発後の長期持続的シナプス減弱におけるスパイン動態 (2013)
  • Author(s): 長谷川翔、大江祐樹、冨永-吉野恵子、小倉明彦
  • Organizer: 日本生理学会
  • Place of Presentation: (東京)
  • Year and Date: 20130300
• [Presentation] 繰り返しLTP・LTD誘発後のシナプス新生・廃止現象とBDNF・proBDNFシグナリング (2012)
  • Author(s): 桜木繁雄、冨永-吉野恵子、小倉明彦
  • Organizer: 生理研研究会
  • Place of Presentation: (岡崎)
  • Year and Date: 20121200
• [Presentation] Involvement of the BDNF-TrkB signaling pathway in the long-lasting synaptic plasticity in hippocampal slice cultures (2012)
  • Author(s): Sakuragi, S., Tominaga-Yoshino, K., Ogura, A.
  • Organizer: Society for Neuroscience
  • Place of Presentation: (New Orleans)
  • Year and Date: 20121100
• [Presentation] 海馬培養切片での長期シナプス可塑性におけるBDNF-TrkB信号経路の関与 (2012)
  • Author(s): 桜木繁雄、冨永-吉野恵子、小倉明彦
  • Organizer: 日本神経科学学会
  • Place of Presentation: (名古屋)
  • Year and Date: 20120900
• [Presentation] 苔状線維-CA3間シナプスで繰り返し長期増強の誘発が引き起こす長期シナプス強化の可能性 (2012)
  • Author(s): 有賀理瑛、谷本浩亀、冨永-吉野恵子、小倉明彦
  • Organizer: 日本神経化学会
  • Place of Presentation: (神戸)
  • Year and Date: 2012-09-10
• [Book] 近藤寿人編「芸術と脳」記憶と夢の仮説 (2013)
  • Author(s): 小倉明彦
  • Publisher: 大阪大学出版会
• [Book] An in vitro model for analyzing the conversion of short-term to long-term memory. In"Short-Term Memory : New Research"(Eds Kalivas, G. & Petralia, S. F.) (2012)
  • Author(s): Tominaga-Yoshino, K. & Ogura, A.
  • Publisher: Nova Science Pub., New York