2013 Fiscal Year Final Research Report
Do synapses distribute uniformly along axonal branches?
| Project/Area Number |
24650208
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tottori University |
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Principal Investigator |
HATA Yoshio 鳥取大学, 医学(系)研究科(研究院), 教授 (40212146)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Takemasa 鳥取大学, 医学部, 助教 (80346345)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | シナプス / 神経回路 / 電気穿孔法 |
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| Research Abstract |
Morphological analysis of axonal projection is commonly used in the research of neural circuitry. To determine whether the density of axonal branches represents functional significance of the projection, I made a plan to map the location of synapses on axonal branches using thalamocortical projections of mice. In the present study, I have developed a novel method to introduce a plasmid into thalamic neurons using in vivo electroporation technique. The present method enables to target a few cells in the deep brain region which is identified physiologically and induce a strong expression of fluorescent proteins enough to visualize entire axonal arborization. Multiple plasmids could be introduced simultaneously using this method. Also, the present method can be applicable not only to mice but also to higher mammals as cats.
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