2014 Fiscal Year Final Research Report
MicroRNA microarray analysis of dopaminergic neurons from FFPE brain sections of patients with Parkinson's disease
| Project/Area Number |
24650222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional brain recording science
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | レーザーマイクロダイセクション / ホルマリン固定パラフィン包埋標本 / microRNA / ドパミン神経細胞 / パーキンソン病 |
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| Outline of Final Research Achievements |
The purpose of this study is to establish miRNA microarray analysis of the dopaminergic neurons that is laser-micro dissected specimens from formalin-fixed, paraffin-embedded (FFPE) tissue sections of patients with Parkinson’s disease. A preliminary experiment using the rat FFPE sections was revealed that the RNA integrity of formalin-fixed dopaminergic neurons is exacerbated by the adoption of the protocol for immunohistochemistry. This poor RNA integrity makes it more difficult to perform miRNA microarray analysis. To improve the RNA integrity, immunohistochemistry protocols may require further modifications.
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| Free Research Field |
神経科学
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