Functional mapping of deep brain structures using microendoscope

2014 Fiscal Year Final Research Report

• Project/Area Number: 24650223
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional brain recording science
• Research Institution: Kyoto University
• Principal Investigator: HAYASHI Yuichiro 京都大学, 医学(系)研究科(研究院), 助教 (90378737)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: カルシウムイメージング / 内視鏡 / 歯状回

Outline of Final Research Achievements

To visualize neural activity in deep brain structures, I developed an endoscope system for fluorescence imaging. Gradient index (GRIN) lens-based endoscope was implanted in a mouse expressing GCaMP7 calcium indicator. The mouse was head-restrained and placed on a spherical treadmill. Using the endoscope, calcium activity of dentate granule cells was visualized. Cellular signals in the fluorescent images were extracted using principal and independent component analysis. Approximately 40 signals were extracted from the images and their signal-to-noise ratio were 4-10.

Free Research Field

神経生理学