Elucidation of cell fate determination mechanism of pancreatic endocrine cells using in vivo imaging

2013 Fiscal Year Final Research Report

• Project/Area Number: 24650228
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: TAKAHASHI Satoru 筑波大学, 医学医療系, 教授 (50271896)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 発生・分化 / 糖尿病 / 細胞・組織 / 応用動物 / 再生医学

Research Abstract

In this study, it is intended to make clear that the fluctuation of the cell fate of pancreatic beta cells is one of the mechanisms of diabetes progression. We also want to reveal that the plasticity of pancreatic endocrine cells associated with the instability is related to insulin resistance and a precipitating factor for the disease. To this end, We want to establish evaluation systems using animal model a new real-time imaging techniques.

Research Products

• [Journal Article] Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination (2014)
  • Author(s): Hasegawa Y, Daitoku Y, Mizuno S, Tanimoto Y, Mizuno-Iijima S, Matsuo M, Kajiwara N, Ema M, Oishi H, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K.
  • Journal Title: Exp Animal Volume: 63 Pages: 183-191
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24770644
  • Peer Reviewed
• [Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination (2013)
  • Author(s): Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K-i.
  • Journal Title: Exp Animal Volume: 62 Pages: 295-304
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Hasegawa+Y%2C+Daitoku+Y%2C+Sekiguchi+K
  • Peer Reviewed
• [Journal Article] In vivo monitoring of pancreaticβ-cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging (2013)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S.
  • Journal Title: Plos One Volume: 8 Pages: e60411
  • DOI: 10.1371/journal.pone.0060411
  • Peer Reviewed
• [Journal Article] Non-invasive monitoring ofβ-cell mass and fetalβ-cell genesis in mouse using bioluminescence imaging (2012)
  • Author(s): Sekiguchi Y, Owada J, Oishi H, Katsumata T, Ikeda R, Kudo T, Takahashi S.
  • Journal Title: Exp Animal Volume: 61 Pages: 445-451
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Sekiguchi+Y%2C+Owada+J%2C+Oishi+H
  • Peer Reviewed
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html