2013 Fiscal Year Final Research Report

Genome-wide screening for the genes essential for the maintenance of mouse embryonic stem cells

Research Project

Project/Area Number	24650236
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Laboratory animal science
Research Institution	Nara Medical University (2013) Osaka University (2012)
Principal Investigator	HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
Project Period (FY)	2012-04-01 – 2013-03-31
Keywords	発生工学 / ES細胞 / ゲノム / 遺伝子 / バイオテクノロジー
Research Abstract	Embryonic stem cells are characterized by unlimited self-renewal capacity and pluripotecy to differentiate into various cell lineages. In depth analyses of these two characteristics are needed in order to apply ES cells toward regenerative medicine. In the present study, we applied gene trap strategy to recently developed mouse haploid ES cells and demonstrated feasibility of gene inactivation through insertional mutageneisis. A large scale application of our mutagenesis scheme would accelerate phenotype-based screen for the genes essential for self-renewal potential and maintenance of pluripotency

(2 results)