Nonliner optical imaging for deep tissue using hollow fiber bundle

2013 Fiscal Year Final Research Report

• Project/Area Number: 24650297
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Medical systems
• Research Institution: Ehime University
• Principal Investigator: YUSUKE Oshima 愛媛大学, 医学部附属病院, 助教(病院教員) (10586639)
• Co-Investigator(Kenkyū-buntansha): KATAGIRI Takashi 東北大学, 大学院工学研究科, 准教授 (90415125)
• Co-Investigator(Renkei-kenkyūsha): IMAMURA Takeshi 愛媛大学, 大学院医学系研究科, 教授 (70264421) ; HIKITA Atsuhiko 愛媛大学, 大学院医学系研究科, 准教授 (60443397)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 深部イメージング / 非線形光学 / 中空ファイバ / 多光子励起蛍光 / 診断システム

Research Abstract

Optical imaging technique with a fluorescent label is a common tool to understand the mechanism of pathogenesis and the vital functions in cellular and molecular level. In this study, We developed a hollow fiber bundle as a basic technique for medical application of the optical imaging that combined deep tissue imaging with nonliner optics such as two-photon excited fluorescence and second harmonic generation (SHG) and endoscopic technique. In the result, the fiber coupling system was constructed and evaluated in biological samples. Two-photon fluorescence images and SHG images of the tissues in mice were successfully obtained using the hollow fiber bundle. These results suggest that the hollow fiber bundle technique is a promising as a practical tool for endoscopic application in the future.

Research Products

• [Journal Article] In vivo imaging of spinal cord in contusion injury model mice by multiphoton microscopy (2014)
  • Author(s): Oshima Y, Horiuchi H, Ogata T, Hikita A, Miura H, Imamura T
  • Journal Title: SPIE Proceedings Volume: Vol. 8947
  • DOI: 10.1117/12.2041042
  • Peer Reviewed
• [Journal Article] Microstructured tube-leaky glass waveguide for delivery of high-powered Er : Yag laser (2014)
  • Author(s): Kobayashi S, Katagiri T, Matsuura Y
  • Journal Title: Journal of Lightwave Technology Volume: 32 (5), art. no. 6693705 Pages: 986-990
  • DOI: 10.1109/JLT.2013.2295838
  • Peer Reviewed
• [Journal Article] Infrared tube-leaky optical fibers with microstructured glass supports (2013)
  • Author(s): Kobayashi S, Katagiri T, Matsuura Y
  • Journal Title: 2013 Optical Fiber Communication Conference and Exposition and the National Fiber Optic Engineers Conference, OFC/NFOEC 2013 Volume: art. no. 6532583
  • Peer Reviewed
• [Presentation] Large-core tube-leaky waveguide for delivery of high-powered Er : YAG laser (2014)
  • Author(s): Kobayashi S, Katagiri T, Matsuura Y
  • Organizer: Photonics West 2014, Biomedical Optics (BIOS 2014) Progress in Biomedical Optics and Imaging, 89381A
  • Place of Presentation: San Francisco, California USA (口頭)
  • Year and Date: 20140201-06
• [Presentation] In vivo imaging of spinal cord in contusion injury model mice by multi-photon microscopy (2014)
  • Author(s): Oshima Y, Horiuchi H, Ogata T, Hikita A, Miura H and Imamura T
  • Organizer: Photonics West 2014, Biomedical Optics (BIOS 2014), Imaging, Manipulation, and Analysis of Biomeleules, Cells, and Tissues XII 8947-7
  • Place of Presentation: San Francisco, California USA (口頭)
  • Year and Date: 20140201-06
• [Presentation] 種々のバイオイメージング技術の構築について (2013)
  • Author(s): 大嶋佑介
  • Organizer: 第11回医用分光学研究会「診断画像構築と増感治療開発の基礎技術としての医用分光」
  • Place of Presentation: 三国観光ホテル(福井県)
  • Year and Date: 20131207-08
  • Invited
• [Presentation] Development of nonlinear optical imaging technique for medical applications by the new optical devises (2013)
  • Author(s): Oshima Y, Katagiri T, Matsuura Y, Hikita A and Imamura T
  • Organizer: 第36回日本分子生物学会年会ワークショップ異分野連携が拓く新規光学デバイスを駆使したバイオイメージングと医療応用への展開」
  • Place of Presentation: ポートピアホテル(兵庫県)
  • Year and Date: 20131203-06
  • Invited
• [Presentation] 赤外イメージング用全誘電体中空光ファイババンドルの提案 (2013)
  • Author(s): 小林駿,片桐崇史,松浦祐司
  • Organizer: 平成25年度電子通信情報学会ソサイエティ大会
  • Place of Presentation: 福岡工業大学(福岡県)
  • Year and Date: 20130917-20
• [Presentation] In vivoイメージングのための非線形光学顕微鏡の開発と医療応用 (2013)
  • Author(s): 大嶋佑介,本蔵直樹,疋田温彦,今村健志
  • Organizer: 第39回レーザ顕微鏡研究会講演会シンポジウム「新規光源を用いた顕微鏡」
  • Place of Presentation: 理化学研究所和光本所(埼玉県)
  • Year and Date: 2013-07-31
  • Invited