Relationship between the contents of high-energy phosphates and Regulation of muscular properties

2012 Fiscal Year Final Research Report

• Project/Area Number: 24650407
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Osaka University
• Principal Investigator: OHIRA Yoshinobu 大阪大学, 大学院・医学系研究科, 教授 (50185378)
• Project Period (FY): 2012
• Keywords: 骨格筋 / 可塑機構 / 遺伝子 / タンパク質 / クレアチンアナログ

Research Abstract

It is known that the properties of skeletal muscles are regulated in response to exercise training, environment, nutrition, growth and development, and/or aging. The energy source for muscle contraction is adenosine triphosphate (ATP), which is synthesized through aerobic and anaerobic energy metabolism. It is also influenced by the content of creatine (AJP Cell Physiol, ’94; Jpn J Physiol, ’94 & ’95). However, the precise mechanism responsible for the regulation of contractile and metabolic properties of muscles is still unclear. Therefore, the current study was performed to investigate the effects of creatine depletion, by feeding creatine analogue, β-guanidinopropionic acid (β-GPA) on the properties of plantaris muscle of C57BL6 mice. Three weeks old male mice were separated into two groups randomly. One group of mice were fed regular powdered diet and other group were supplied the same diet, but containing 1% β-GPA. The amount of food, pair -fed, was gradually increased following growth, but 1 g of food was given to each mouse daily after 3 weeks. Water was supplied ad libitum. At 4th and 8th week, blood and muscle samplings were performed. Blood was withdrawn from the jugular vein under anesthesia with i.p. injection of sodium pentobarbital. Serum was saved after centrifugation and creatine content was analyzed. Plantaris muscles were sampled bilaterally. Muscle fiber types were analyzed in the cross-section of right muscles. Gene expressions were determined in the left muscles. Significant depletion of creatine and shift of fibers toward slow-type were noted, especially after 8 weeks of β-GPA feeding. Increase in the expression of genes related to slow-type fibers and mitochondrial metabolism was noted even at 4th week. It is suggested that depletion of creatine causes the transformation of muscle fibers toward slow-twitch type.

Research Products

• [Journal Article] HSP25 can modulate myofiber desmin cytoskeleton following the phosphorylation at Ser15 in rat soleus muscle (2012)
  • Author(s): Kawano, F., R. Fujita, N. Nakai, M. Terada, T. Ohira, and Y. Ohira
  • Journal Title: J. Appl. Physiol Volume: 112 Pages: 176-186
• [Journal Article] (2012)
  • Author(s): Arima, Y., M. Harada, D. Kamimura, J-H. Park, F. Kawano, F.E. Yull. T. Kawamoto, Y. Iwakura, U.A.K. Betz, G. Marquez, T.S. Blackwell, Y. Ohira, T. Hirano, and M. Murakami.
  • Journal Title: Cell Volume: 148 Pages: 447-457
• [Journal Article] Effects of mechanical over-loading on the properties of soleus muscle fibers, with or without damage, in wild type and mdx mice (2012)
  • Author(s): Terada, M., F. Kawano, T. Ohira, N. Nakai, N. Nishimoto, and Y. Ohira
  • Journal Title: PLoS ONE Volume: 7 (4) Pages: e34557
• [Journal Article] Biomedical analysis of rat body hair after hindlimb suspension for 14 days. (2012)
  • Author(s): Terada, M., F. Kawano, N. Ishioka, A. Higashibata, H. J. Majima, T. Yamazaki, T. Watanabe-Asaka, M. Niihori, R. Nakao, S. Yamada, C. Mukai, and Y. Ohira
  • Journal Title: Acta Astronautica Volume: 73 Pages: 23-29
• [Journal Article] Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats (2012)
  • Author(s): Nomura, S., K. Kami, F. Kawano, Y. Oke, N. Nakai, T. Ohira, R. Fujita, M. Terada, K. Imaizumi, and Y. Ohira
  • Journal Title: Neurosci. Lett. Volume: 509 Pages: 76-81
• [Journal Article] Adaptation of mouse skeletal muscle to long-term microgravity in the MDS mission (2012)
  • Author(s): Sandona, D., J.-F. Desaphy, G.M. Camerino, E. Bianchini, S. Ciciliot, D. Danieli-Betto, G. Dobrowolny, S. Furlan, E. Germinario, K. Goto, M. Gutsmann, F. Kawano, N. Nakai, T. Ohira, Y. Ohno, A. Picard, M. Salanova, G. Schiffl, D. Blottner, A. Musaro, Y. O
  • Journal Title: PLoS ONE Volume: 7(3) Pages: e33232
• [Journal Article] The impact of long-term exposure to space environment on adult mammalian organisms: a study on mouse thyroid and testis. (2012)
  • Author(s): Masini, M.A., E. Albi, C. Barmo, T. Bonfiglio, L. Bruni, L. Canesi, S. Cataldi, F. Curcio, M. D'Amora, I. Ferri, K. Goto, F. Kawano, R. Lazzarini, E. Loreti, N. Nakai, T. Ohira, Y. Ohira, S. Palmero, P. Prato, F. Ricci, L. Scarabelli, T. Shibaguchi, R. Sp
  • Journal Title: PLoS ONE Volume: 7(4) Pages: e35418
• [Journal Article] Evaluation of gene, protein and neurotrophin expression in the brain of mice exposed to space environment for 91 days. (2012)
  • Author(s): Santucci, D., F. Kawano, T. Ohira, M. Terada, N. Nakai, N. Francia, E. Alleva, L.Aloe, T. Ochiai, R. Cancedda, K. Goto,and Y. Ohira
  • Journal Title: PLoS ONE Volume: 7(7) Pages: e40112
• [Journal Article] Regulation of soleus muscle properties by mechanical stress and/or neural activity. (2012)
  • Author(s): Kawano, F., N. Nakai, and Y. Ohira
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 (1) Pages: 29-36
• [Journal Article] Some aspects of heat stress on the plasticity of skeletal muscle cells (2012)
  • Author(s): Goto, K., Y. Ohno, A. Goto, A. Ikuta, M. Suzuki, T . Ohira, N. Tsuchiya, S. Nishizawa, T . Koya, T. Egawa, T. Sugiura, Y . Ohira, and T. Yoshioka
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 (2) Pages: 197-204
• [Journal Article] Control of muscle protein synthesis in response to exercise and amino acids. (2012)
  • Author(s): Nakai, N., F. Kawano, and Y. Ohira
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 (2) Pages: 297-305
• [Presentation] Responses of gene and protein expressions in mouse neck muscle to micro- and/or hyper-gravity environment. (2012)
  • Author(s): T omo. Ohira, T ak. Ohira, F. Kawano, H. Okabe, N. Nakai, T. Ochiai, K. Goto, Y. Ohno, R. Cancedda, and Y. Ohira
  • Organizer: 28th Annual Meeting of the American Society for Gravitational and Space Research
  • Place of Presentation: New Orleans, Louisiana, U.S.A
  • Year and Date: 20121128-1202
• [Presentation] Responses of rat soleus muscle to mechanical stress and /or high-energy phosphate contents. (2012)
  • Author(s): H. Okabe,T omo. Ohira, T ak. Ohira, F. Kawano, K. Goto, H.Naito, and Y. Ohira
  • Organizer: 28th Annual Meeting of the American Society for Gravitational and Space Research.
  • Place of Presentation: New Orleans, Louisiana, U.S.A
  • Year and Date: 20121128-1202
• [Presentation] ラット後肢筋における機械的刺激と高エネルギーリン酸レベルの関係. (2012)
  • Author(s): 岡部洋興、大平友宇、大平宇志、河野史倫、芝口翼、後藤勝正、内藤久士、大平充宣
  • Organizer: 第58回日本宇宙航空環境医学会大会
  • Place of Presentation: 豊橋商工会議所
  • Year and Date: 20121115-17
• [Presentation] 重力レベルがマウス頸筋におけるタンパク質発現に及ぼす影響 (2012)
  • Author(s): 大平友宇、大平宇志、河野史倫、芝口翼、岡部洋興、大野善隆、須藤正道、後藤勝正、Ranieri Cancedda、大平充宣
  • Organizer: 第58回日本宇宙航空環境医学会大会
  • Place of Presentation: 豊橋商工会議所
  • Year and Date: 20121115-17
• [Presentation] 骨格筋の量的変化に伴う細胞内脂肪とその分布 (2012)
  • Author(s): 大平友宇、鈴木美穂、後藤亜由美、生田旭洋、大野善隆、古屋智之、西澤苑、江川達郎、杉浦崇夫、大平充宣、吉岡利忠、後藤勝正
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜長良川国際会議場
  • Year and Date: 20120914-16
• [Presentation] マウスヒラメ筋の量的変化に伴う sirtuin 発現の変化 (2012)
  • Author(s): 後藤亜由美、大野善隆、江川達郎、大平友宇、鈴木美穂、生田旭洋、西澤苑、古屋智之、杉浦崇夫、大平充宣、吉岡利忠、後藤勝正
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜長良川国際会議場
  • Year and Date: 20120914-16
• [Presentation] 骨格筋の萎縮と肥大に伴うミオシン重鎖発現と熱ショック転写因子 1 (2012)
  • Author(s): 古屋智之、西澤苑、別府諸兄、大平充宣、吉岡利忠、杉浦崇夫、大平友宇、鈴木美穂、後藤亜由美、生田旭洋、唐柳林、江川達郎、大野善隆、後藤勝正
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜長良川国際会議場.
  • Year and Date: 20120914-16
• [Presentation] 損傷骨格筋の再生における熱ショック転写因子 1の生理学的意義 (2012)
  • Author(s): 西澤苑、古屋智之、大野善隆、江川達郎、大平友宇、鈴木美穂、後藤亜由美、生田旭洋、杉浦崇夫、大平充宣、吉岡利忠、別府諸兄、後藤勝正
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜長良川国際会議場
  • Year and Date: 20120914-16
• [Presentation] Responses of myosin heavy chain phenotypes and gene expressions in neck muscle to micro- and hyper-gravity in mice. (2012)
  • Author(s): Tomo. Ohira, Tak. Ohira, F . Kawano, T. Shibaguchi, H. Okabe, N. Nakai, T. Ochiai, K. Goto, and Y . Ohira
  • Organizer: 12th European Life Sciences Symposium and 33rd Annual International Gravitational Physiology Meeting
  • Place of Presentation: Aberdeen, United Kingdom (Scotland)
  • Year and Date: 20120618-22
• [Presentation] Recording of neuromuscular activities of rats in a real microgravity environment using telemetry system. (2012)
  • Author(s): Kawano, F., T. Ohira, T. Ochiai, and Y. Ohira
  • Organizer: Next-generation suborbital researchers conference
  • Place of Presentation: Calif., U.S.A
  • Year and Date: 20120505-08
• [Presentation] Expectation for suborbital flight as life science experiment opportunity . (2012)
  • Author(s): Ochiai, T., H. Murase, and Y. Ohira
  • Organizer: Next-generation suborbital researchers conference.
  • Place of Presentation: Calif., U.S.A
  • Year and Date: 20120505-08